frequency of quinolone resistance and qnrb and qnrc genes in clinical isolates of klebsiella pneumoniae

نویسندگان

maryam moghadasi department of microbiology, boroujerd branch, islamic azad university, boroujerd, iran.

mohsen mirzaee department of medical laboratory sciences, boroujerd branch, islamic azad university, boroujerd, iran.

mohammad reza mehrabi department of medical laboratory sciences, boroujerd branch, islamic azad university, boroujerd, iran.

چکیده

background :    klebsiella pneumoniae is among the most common and significant agents of community and hospital-acquired infections. plasmid mediated quinolone resistance (pmqr) was increasingly identified in enterobacteriaceae family worldwide. plasmid-mediated quinolone resistance (pmqr) was first reported in 1998 from a klebsiella pneumoniae isolate in the usa. till date, five qnr proteins have been identified; qnra, qnrb, qnrc, qnrd and qnrs. quinolone are broad spectrum antibiotics the resistant to which has increasingly been reported among many bacterial species including klebsiella . the aim of this study was the antibiotic resistance profile was determined based on resistance and molecular characterization of qnrb and qnrc genes in klebsiella pneumoniae clinical isolated. methods :   in this cross sectional study, 94 samples of k. pneumoniae were collected. isolates were screened for quinolone antibiotics resistance using disk diffusion method according to clinical and laboratory standards institute (clsi) guidelines. isolates with resistance to at least one of the quinolone antibiotics, examined for presence of the qnrb and qnrc genes. results :   based on the obtained results by the agar disk diffusion test, 29.78%, 27.65%, 28.72% and 27.65% of the isolates were resistant to nalidixic acid, ciprofloxacin, norfloxacin and ofloxacin, respectively. of these 46.66% carried qnrb , 3.33% carried qnrc genes.   conclusion :   the identification of qnrb gene among quinolone-resistant k. pneumoniae isolates shows that the emergence of pmqr in this region requires serious preventive measures.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The Emergence of Qnr-resistance Among Klebsiella pneumoniae spp in Zahedan

Objective: Although quinolone resistance results mostly from chromosomal mutations in Klebsiella pneumoniae, it may also be mediated by plasmid – encoded qnr determinants. Plasmid-mediated quinolone resistance (PMQR) was increasingly identified in Enterobacteriaceae family worldwide. The aim of this study was to investigate the prevalence of qnr genes in clinical isolates of Klebsiella pn...

متن کامل

Correlation of quinolone-resistance, qnr genes and integron carriage in multidrug-resistant community isolates of Klebsiella spp.

Objective(s): Plasmid-mediated quinolone resistance (PMQR) determinants and integrons have a considerable contribution to bacterial drug resistance in Gram-negative pathogens. We studied the prevalence of PMQR genes and integron carriage in multidrug-resistant community isolates of Klebsiella spp.Materials and Methods: Two hundred and fi...

متن کامل

Evaluation of Antibiotic Resistance Patterns of Clinical Klebsiella pneumoniae Isolates from Educational Hospitals in Zahedan, Iran

ABSTRACT Background and Objectives: The resistance of gram-negative bacteria to antibiotics has become a serious problem, which imposes a significant increase in treatment costs. Klebsiella pneumoniae is an important nosocomial pathogen from the Enterobacteriaceae family. The aim of this study was to investigate the frequency and pattern of antibiotic resistance in K. pneumoniae strains isolat...

متن کامل

The main purpose of this study was the pattern of antibiotic resistance and the frequency of plasmid-dependent quinolone resistance (qnr) genes in Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae isolates isolated from surgical site

Introduction: In recent years, antibiotic resistance has been on the rise, leading to limited ways to control nosocomial infections (especially surgical site infections) and the right treatment options. The main purpose of this study was the pattern of antibiotic resistance and the frequency of plasmid-dependent quinolone resistance (qnr) genes in Pseudomonas aeruginosa, Escherichia coli and Kl...

متن کامل

Presence of quinolone resistance to qnrB1 genes and blaOXA-48 carbapenemase in clinical isolates of Klebsiella pneumoniae in Spain.

A study is presented on the presence of quinolone resistance qnrB1 genes in clinical isolates belonging to the largest series of infections caused by OXA-48-producing Klebsiella pneumoniae in a single-centre outbreak in Spain. Evidence is also provided, according to in vitro results, that there is a possibility of co-transfer of plasmid harbouring blaOXA-48 with an other plasmid harbouring qnrB...

متن کامل

Prevalence of qnr and aac(6’)-Ib-cr Genes in Clinical Isolates of Klebsiella Pneumoniae from Imam Hussein Hospital in Tehran

Background: Plasmid mediated quinolone resistance (PMQR) has been shown to play an important role in resistance not only to quinolones, but also β-lactams and aminoglycosides. In fact, qnr genes are frequently carried along with β-lactamase determinants on the same plasmids. We studied the prevalence of qnrA, qnrB, qnrS and aac(6’)-Ib-cr genes among quinolone and cephalosporin resistant clinica...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
journal of medical bacteriology

جلد ۵، شماره ۵-۶، صفحات ۳۹-۴۵

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023